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bronchial epithelial cell growth kit  (ATCC)


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    ATCC bronchial epithelial cell growth kit
    Bronchial Epithelial Cell Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bronchial+epithelial+cell+growth+kit/Bronchial+Epithelial+Cell+Growth+Kit/pm42773388-35-13-19
    Average 96 stars, based on 298 article reviews
    bronchial epithelial cell growth kit - by Bioz Stars, 2026-10
    96/100 stars

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    Cell Culture:

    Article Title: IL-8 drives EMT and malignant progression in chronic obstructive pulmonary disease-associated lung cancer via STAT3 signaling.
    Article Snippet: .. The human bronchial epithelial cell line HBEC3-KT (ATCC, Manassas, VA, USA) was cultured in Airway Epithelial Cell Basal Medium supplemented with a Bronchial Epithelial Cell Growth Kit (ATCC) and 1% penicillin/streptomycin (Gibco, Waltham, MA, USA). .. The human lung adenocarcinoma cell lines A549 (ATCC) and 95D (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, China) were maintained in Dulbecco’s modified Eagle medium (DMEM) GlutaMAXTM (Gibco) or RPMI-1640 medium (Gibco), respectively, supplemented with 10% FBS and 1% penicillin/streptomycin.

    Article Title: TAD boundaries and gene activity are uncoupled
    Article Snippet: For transcription inhibition, HBECs and HFF cells were treated with 100 μM DRB (Sigma-Aldrich, cat. no. D1916) in culture medium for 2 hr, then fixed as described below. .. For glucocorticoid-mediated transcriptional stimulation, HBECs were cultured in Airway Epithelial Cell Basal Medium (ATCC, cat. no. PCS-300-030) supplemented with the Bronchial Epithelial Cell Growth Kit (ATCC, cat. no. PCS-300-040) and penicillin/streptomycin. .. To eliminate background glucocorticoid activity, cells were transferred 24 hr before induction to hormone-free medium, prepared by supplementing the basal ATCC Airway Epithelial Medium with HLL Supplement, L-glutamine, and penicillin/streptomycin while omitting both the P-extract and the Airway Epithelial Cell Supplement, as these components contain glucocorticoids.

    Article Title: Trifluoperazine causes mast cell apoptosis through a secretory granule-mediated pathway
    Article Snippet: Primary human lung fibroblasts (HLFs) (PCS-201-013) and primary human lung smooth muscle cells (HLSMCs)(PCS-130-010) were obtained from American Type Culture Collection (ATCC, Manassas, VA) and cultured following the manufacturer’s instructions. .. Primary human small airway epithelial cells (HSAECs) (PCS-301-010) were obtained from ATCC and cultured in Airway Epithelial Cell Basal Medium (ATCC) containing a Bronchial Epithelial Cell Growth Kit (ATCC) and penicillin-streptomycin (100 U/mL, 100 μg/mL). .. Triple-negative human breast cancer cell line Hs578T was obtained from ATCC (HTB-126) and cultured at 37 °C in 5% CO 2 in DMEM (Invitrogen) supplemented with 10% FBS, penicillin-streptomycin (50 μg/ml, 60 μg/ml), and 2 mM L-glutamine (Sigma-Aldrich).

    Article Title: Trifluoperazine causes mast cell apoptosis through a secretory granule-mediated pathway.
    Article Snippet: Primary human lung fibroblasts (HLFs) (PCS-201-013) and primary human lung smooth muscle cells (HLSMCs)(PCS-130-010) were obtained from American Type Culture Collection (ATCC, Manassas, VA) and cultured following the manufacturer’s instructions. .. Primary human small airway epithelial cells (HSAECs) (PCS-301-010) were obtained from ATCC and cultured in Airway Epithelial Cell Basal Medium (ATCC) containing a Bronchial Epithelial Cell Growth Kit (ATCC) and penicillin-streptomycin (100 U/mL, 100 μg/mL). .. Immortalized Human Mast Cell Line (LUVA) was obtained from Kerafast (#EG1701-FP) and cultured in complete StemPro-34 SFM (Thermo Fisher Scientific), supplemented with 2mM L-glutamine or 1× GlutaMAX (Thermo Fisher Scientific) and penicillin–streptomycin in the presence of IL-3, SCF, and IL-6 (Peprotech).

    Article Title: Hypomethylation of FAM83A in lung adenocarcinoma mirrors an epigenetic signature of airway cell differentiation states.
    Article Snippet: .. The human bronchial epithelial cell line HBEC3-KT (CRL-4051, ATCC, Manassas, VA, USA) was cultured in Airway Epithelial Cell Basal Medium (PCS-300-030, ATCC, Manassas, VA, USA) supplemented with the Bronchial Epithelial Cell Growth Kit (PCS-300-040, ATCC, Manassas, VA, USA). .. The immortalized foreskin fibroblast cell line HFF2-hTERT (kindly provided by Dr Decottignies, de Duve Institute, UCLouvain) was maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, St-Louis, MO, USA) and 1% penicillinstreptomycin (Life Technologies, Carlsbad, CA, USA).



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    (A) Transwell plates coated with human bronchial <t>epithelial</t> cells were subject to infection with pre-treated pneumococcal bacteria, where the number of bacteria per mL (cfu/mL) is graphed for each time point tested. (B-C) Mice were challenged with WU2 (serotype 3) S. pneumoniae, and (B) lung and (C) blood bacterial titers were performed at 24, 48, and 72 hours post-infection. (n=10 mice per group). ns, non-significant; *, p ≤ 0.05, ** p ≤ 0.01; ***, p ≤ 0.001; **** p ≤ 0.0001.
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    (A) Transwell plates coated with human bronchial epithelial cells were subject to infection with pre-treated pneumococcal bacteria, where the number of bacteria per mL (cfu/mL) is graphed for each time point tested. (B-C) Mice were challenged with WU2 (serotype 3) S. pneumoniae, and (B) lung and (C) blood bacterial titers were performed at 24, 48, and 72 hours post-infection. (n=10 mice per group). ns, non-significant; *, p ≤ 0.05, ** p ≤ 0.01; ***, p ≤ 0.001; **** p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: A pan-serotype human monoclonal antibody protects against pneumococcal infection by targeting multiple choline binding domain proteins

    doi: 10.64898/2026.05.28.728567

    Figure Lengend Snippet: (A) Transwell plates coated with human bronchial epithelial cells were subject to infection with pre-treated pneumococcal bacteria, where the number of bacteria per mL (cfu/mL) is graphed for each time point tested. (B-C) Mice were challenged with WU2 (serotype 3) S. pneumoniae, and (B) lung and (C) blood bacterial titers were performed at 24, 48, and 72 hours post-infection. (n=10 mice per group). ns, non-significant; *, p ≤ 0.05, ** p ≤ 0.01; ***, p ≤ 0.001; **** p ≤ 0.0001.

    Article Snippet: Cells were maintained in complete growth medium consisting of airway epithelial cell basal medium (ATCC Cat #PCS 300-030) supplemented with a bronchial epithelial cell growth kit (ATCC Cat #PCS 300-040) , penicillin (10 U/mL), streptomycin (10 mg/mL), and amphotericin B (25 μg/mL) (ATCC Cat # PCS-999-002).

    Techniques: Infection, Bacteria